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Importantly, although the usage of higher doses of AAA promoted the brain entry, it had undesirable side effects, as cerebral microhemorrhage (Wilcock et al., 2004)

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Effects of treadmill exercise on the regulatory mechanisms of mitochondrial dynamics and oxidative stress in the brains of high-fat diet fed rats

The cells were then washed twice with PBS and then overlaid with cystine-free RPMI supplemented with 20 nM Sytox Green (Fisher Scientific, S7020) and the indicated treatment